1) BACTERICIDAL ACTIVITY (TESTS CARRIED OUT IN EUROPE)

Testing the neutral anolyte against two bacterial isolates from the uterus of mares.

 

 

a ) - Streptococcus zooepidemicus

The microbial activity of the neutral anolyte was examined against methicillin sensory: Staphylococcus Aureus, Staphylococcus epermidis, Serratia marcescens, Escherichia coli, Pseudomonas aeruginosa and Burkholderia Cepacia which are serious pathogens.

The bactericidal properties of the neutral anolyte were evaluated with three conventional disinfectants which are: Chlorhexidine at 0.1%, povidone iodine 0.02% and 80% ethanol. The concentrations chosen represent those generally used in the solutions prepared for washing hands. All disinfecting solutions were mixed with sterile distilled water at the time of their use. The sterile distilled water was used as control. The results are summarized in Tables 1 and 2.

 

 

The ANAQUA units and neutral anolyte products have been tested by large series of independent tests and on site trials, including hospitals, breweries, water treatment systems, farms of animals and agricultural environments.

b) - Escherichia coli

Bacterial counts

Bacterial counts

Total viable count of inoculum> 5000 cfu / ml

Total viable count of inoculum> 5000 cfu / ml

Microbiological Performance

Independent testing

Exposure Time

Dilution

30 s

1 min

5 min

10min

20 min

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

10

2

10

3

10

4

10

5

Exposure

Dilution

30 s

1 min

5 min

10min

20 min

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

10

2

10

3

10

5

10

4

Table 1. Bactericidal effect of neutral anolyte on an  inoculum of  1.7 x 104 cfu/mL

 Trial 1

TRIALS

 MADE

 ON SITES

Being environmentally friendly, the neutral anolyte is completely safe, non-toxic and non-irritating. It can be used where traditional chemicals do not produce the desired results or where traditional products cannot be used.

The findings show that the neutral anolyte is an inexpensive and powerful disinfectant with many benefits to be recommended in multiple solution, sterilization procedures, disinfection and water purification.

A safer world

The alternative solution

Trial 2

Table 2. Bactericidal effect anolyte inoculum of  1.7 x 106 cfu/mL

 

Bacteria

Disinfectant

10 s

60 s

180 s

Contact time

MSSA methicillin sensitive Staphylococcus aureus

MRSA methicillin resistant Staphylococcus aureus

Staphylococcus epedermis

Pseudomonas aeruginosa

Escherichia coli

Serratia marcescens

Burkholderia cepacia

Neutral anolyte

0.02% Povidone iodine

80% Ethanol

0.1% chlorhexidine

Control: distilled water

Neutral anolyte

0.02% Povidone iodine

80% Ethanol

0.1% chlorhexidine

Control: distilled water

Neutral anolyte

0.02% Povidone iodine

80% Ethanol

0.1% chlorhexidine

Control: distilled water

Neutral anolyte

0.02% Povidone iodine

80% Ethanol

0.1% chlorhexidine

Control: distilled water

Neutral anolyte

0.02% Povidone iodine

80% Ethanol

0.1% chlorhexidine

Control: distilled water

Neutral anolyte

0.02% Povidone iodine

80% Ethanol

0.1% chlorhexidine

Control: distilled water

Neutral anolyte

0.02% Povidone iodine

80% Ethanol

0.1% chlorhexidine

Control: distilled water

0

0

0

>500

>500

0

0

0

>500

>500

0

0

0

>500

>500

0

0

0

>27

>500

0

0

0

>500

>500

0

0

0

0

>500

0

0

0

0

>500

0

0

0

>500

>500

0

0

0

>500

>500

0

0

0

>500

>500

0

0

0

>500

>500

0

0

0

>500

>500

0

0

0

41

>500

0

0

0

0

>500

0

0

0

>500

>500

0

0

0

>500

>500

0

0

0

0

>500

0

0

0

0

>500

0

0

0

>500

>500

0

0

0

0

>500

0

0

0

0

>500

MSSA methicillin sensitive Staphylococcus aureus

Neutral anolyte

0.02% Povidone iodine

80% Ethanol

0.1% chlorhexidine

Control: distilled water

MRSA methicillin resistant Staphylococcus aureus

Neutral anolyte

0.02% Povidone iodine

80% Ethanol

0.1% chlorhexidine

Control: distilled water

Staphylococcus epedermis

Neutral anolyte

0.02% Povidone iodine

80% Ethanol

0.1% chlorhexidine

Control: distilled water

Pseudomonas aeruginosa

Neutral anolyte

0.02% Povidone iodine

80% Ethanol

0.1% chlorhexidine

Control: distilled water

Escherichia coli

Neutral anolyte

0.02% Povidone iodine

80% Ethanol

0.1% chlorhexidine

Control: distilled water

Serratia marcescens

Neutral anolyte

0.02% Povidone iodine

80% Ethanol

0.1% chlorhexidine

Control: distilled water

Burkholderia cepacia

Neutral anolyte

0.02% Povidone iodine

80% Ethanol

0.1% chlorhexidine

Control: distilled water

Bacteria

Disinfectant

0

8

0

>500

>500

 

0

15

0

>500

>500

 

0

0

0

>500

>500

0

0

0

>500

>500

3

>500

0

>500

>500

 

0

0

0

>500

>500

 

0

71

0

>500

>500

 

0

0

0

>500

>500

0

0

0

>500

>500

0

0

0

>500

>500

0

0

0

>27

>500

0

237

0

>500

>500

 

0

0

0

>500

>500

0

0

0

1

>500

0

0

0

>500

>500

0

0

0

>500

>500

0

0

0

>500

>500

0

0

0

0

>500

0

0

0

>500

>500

0

0

0

>500

>500

0

0

0

0

>500

10 s

60 s

180 s

Contact time

Conclusion : The number of bacteria was reduced below the detection limit after 10 seconds incubation in neutral anolyte. The bactericidal activity of the neutral anolyte was similar to that of ethanol at 80%, but greater than 0.1% chlorhexidine and 0.02% iodine Povidone. We conclude that the anolyte neutral is a powerful, cost-effective disinfectant.

Trial 3

Microorganism

Inoculum concentration

Contact time

Escherichia coli

1, 5, 10 et 30 minutes

Salmonella sp.

1, 5, 10 et 30 minutes

Pseudomonas aeruginosa

5, 10, 30 et 60 minutes

Pneumophila legionella

5, 10, 30 et 60 minutes

1,5 x 10  cfu/ml

5

Parameter

Units

Initial

values

1 min.

5 min

10 min.

30 min.

pH

PH unit

8,1

8,1

8,1

8,1

8,0

Residual chlorine

Units

mg/l

0,7

0,7

0,7

0,7

Redox potential

mV

825

-

-

-

-

Escherichia coli

cfu / 100ml

1,5 x 10

0

0

0

0

Heterotrophic

bacteria

cfu / ml

< 1

-

-

-

-

1,3 x 10  cfu/ml

5

1,1 x 10  cfu/ml

5

1,5 x 10  cfu/ml

5

Tests of the neutral anolyte against Escherichia coli, Salmonella species, Pseudomonas aeruginosa and Legionella pneumophila

Testing Results

Table 1

Neutral anolyte + Bacteria

5

Table 2

Parameter

Units

Initial

values

1 min.

5 min

10 min.

30 min.

pH

PH unit

8,1

8,1

8,1

8,1

8,0

Residual chlorine

Units

mg/l

0,7

0,7

0,7

0,7

Redox potential

mV

825

-

-

-

-

Salmonella spp.

cfu / 100ml

1,3 x 10

NEG

NEG

NEG

NEG

Heterotrophic

bacteria

cfu / ml

< 1

-

-

-

-

Neutral anolyte + Bacteria

5

Table 3

Parameter

Units

Initial

values

1 min.

5 min

10 min.

30 min.

pH

PH unit

8,1

8,1

8,1

8,1

8,0

Residual chlorine

Units

mg/l

0,7

0,7

0,7

0,7

Redox potential

mV

825

-

-

-

-

Pseudomonas aeruginosa

cfu / 100ml

1,1 x 10

POS

NEG

NEG

NEG

Heterotrophic

bacteria

cfu / ml

< 1

150

< 1

< 1

< 1

Neutral anolyte + Bacteria

5

Table 4

Parameter

Units

Initial

values

1 min.

5 min

10 min.

30 min.

pH

PH unit

8,1

8,1

8,1

8,1

8,0

Residual chlorine

Units

mg/l

0,7

0,7

0,7

0,7

Redox potential

mV

825

-

-

-

-

Legionella

pneumophila

cfu / 100ml

1,5 x 10

1200

200

0

0

Heterotrophic

bacteria

cfu / ml

< 1

1200

200

< 1

< 1

Neutral anolyte + Bacteria

5

CONCLUSION : This study shows that the disinfecting action of the neutral anolyte is fast and efficient. It takes one minute to eradicate E. coli and Salmonella sp. and, after 10 minutes, no more of the tested strains are present.

 

 

2) SPORICIDAL ACTIVITY

Remaining spores (log10), after exposure to the neutral anolyte or 2% glutaraldehyde

 

Contact Time

Anolyte Neutral

 

2% glutaraldehyde

Pre - disinfection challenge

 

7,76

 

7,76

1 min

 

4,84

 

7,63

2 min

 

2,34

 

7,60

5 min

 

1,30

 

7,46

10 min

 

0

 

7,19

20 min

 

0

 

6,87

30 min

 

0

 

6,34

1 heure

 

0

 

2,75

2 heures

 

0

 

0

 

CONCLUSION : This study shows that the neutral anolyte produced using ANAQUA units is highly effective as a sporicidal agent. A reduction of 6log10 spores with a freshly produced anolyte neutral solution was observed in 5 minutes.